2,208 research outputs found
Raft Instability of Biopolymer Gels
Following recent X-ray diffraction experiments by Wong, Li, and Safinya on
biopolymer gels, we apply Onsager excluded volume theory to a nematic mixture
of rigid rods and strong ``'' cross-linkers obtaining a long-ranged,
highly anisotropic depletion attraction between the linkers. This attraction
leads to breakdown of the percolation theory for this class of gels, to
breakdown of Onsager's second-order virial method, and to formation of
heterogeneities in the form of raft-like ribbons.Comment: 5 pages, 4 figure
Electrostatic complexation of spheres and chains under elastic stress
We consider the complexation of highly charged semiflexible polyelectrolytes
with oppositely charged macroions. On the basis of scaling arguments we discuss
how the resulting complexes depend on the persistence length of the
polyelectrolyte, the salt concentration, and the sizes and charges of the chain
and the macroions. We study first the case of complexation with a single sphere
and calculate the wrapping length of the chain. We then extend our
considerations to complexes involving many wrapped spheres and study
cooperative effects. The mechanical properties of such a complex under an
external deformation are evaluated.Comment: 16 pages, submitted to J. Chem. Phy
Continuous sedation until death: The everyday moral reasoning of physicians, nurses and family caregivers in the UK, The Netherlands and Belgium
Copyright © 2014 Raus et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative
Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly credited.Background - Continuous sedation is increasingly used as a way to relieve symptoms at the end of life. Current research indicates that some physicians, nurses, and relatives involved in this practice experience emotional and/or moral distress. This study aims to provide insight into what may influence how professional and/or family carers cope with such distress.
Methods - This study is an international qualitative interview study involving interviews with physicians, nurses, and relatives of deceased patients in the UK, The Netherlands and Belgium (the UNBIASED study) about a case of continuous sedation at the end of life they were recently involved in. All interviews were transcribed verbatim and analysed by staying close to the data using open coding. Next, codes were combined into larger themes and categories of codes resulting in a four point scheme that captured all of the data. Finally, our findings were compared with others and explored in relation to theories in ethics and sociology.
Results - The participants’ responses can be captured as different dimensions of ‘closeness’, i.e. the degree to which one feels connected or ‘close’ to a certain decision or event. We distinguished four types of ‘closeness’, namely emotional, physical, decisional, and causal. Using these four dimensions of ‘closeness’ it became possible to describe how physicians, nurses, and relatives experience their involvement in cases of continuous sedation until death. More specifically, it shined a light on the everyday moral reasoning employed by care providers and relatives in the context of continuous sedation, and how this affected the emotional impact of being involved in sedation, as well as the perception of their own moral responsibility.
Conclusion - Findings from this study demonstrate that various factors are reported to influence the degree of closeness to continuous sedation (and thus the extent to which carers feel morally responsible), and that some of these factors help care providers and relatives to distinguish continuous sedation from euthanasia.The Economic and Social Research
Council (UK), the Research Foundation Flanders
(BE), the Flemish Cancer Association (BE), the Research Council of Ghent
University (BE), the Netherlands Organization for Scientific Research (NL) and
the Netherlands Organization for Health Research and Development (NL)
DNA Spools under Tension
DNA-spools, structures in which DNA is wrapped and helically coiled onto
itself or onto a protein core are ubiquitous in nature. We develop a general
theory describing the non-equilibrium behavior of DNA-spools under linear
tension. Two puzzling and seemingly unrelated recent experimental findings, the
sudden quantized unwrapping of nucleosomes and that of DNA toroidal condensates
under tension are theoretically explained and shown to be of the same origin.
The study provides new insights into nucleosome and chromatin fiber stability
and dynamics
Structural Polymorphism of the Cytoskeleton: A Model of Linker-Assisted Filament Aggregation
The phase behavior of charged rods in the presence of inter-rod linkers is
studied theoretically as a model for the equilibrium behavior underlying the
organization of actin filaments by linker proteins in the cytoskeleton. The
presence of linkers in the solution modifies the effective inter-rod
interaction and can lead to inter-filament attraction. Depending on the
system's composition and physical properties such as linker binding energies,
filaments will either orient perpendicular or parallel to each other, leading
to network-like or bundled structures. We show that such a system can have one
of three generic phase diagrams, one dominated by bundles, another by networks,
and the third containing both bundle and network-like phases. The first two
diagrams can be found over a wide range of interaction energies, while the
third occurs only for a narrow range. These results provide theoretical
understanding of the classification of linker proteins as bundling proteins or
crosslinking proteins. In addition, they suggest possible mechanisms by which
the cell may control cytoskeletal morphology.Comment: 17 pages, 3 figure
Polymer reptation and nucleosome repositioning
We consider how beads can diffuse along a chain that wraps them, without
becoming displaced from the chain; our proposed mechanism is analogous to the
reptation of "stored length" in more familiar situations of polymer dynamics.
The problem arises in the case of globular aggregates of proteins (histones)
that are wound by DNA in the chromosomes of plants and animals; these beads
(nucleosomes) are multiply wrapped and yet are able to reposition themselves
over long distances, while remaining bound by the DNA chain.Comment: 9 pages, including 2 figures, to be published in Phys. Rev. Let
Screened and Unscreened Phases in Sedimenting Suspensions
A coarse-grained stochastic hydrodynamical description of velocity and
concentration fluctuations in steadily sedimenting suspensions is constructed,
and analyzed using self-consistent and renormalization group methods. We find
that there exists a dynamical, non-equilibrium phase transition from an
"unscreened" phase in which we recover the Caflisch-Luke (R.E. Caflisch and
J.H.C. Luke, Phys. Fluids 28, 759 (1985)) divergence of the velocity variance
to a "screened" phase where the velocity fluctuations have a finite correlation
length growing as where is the particle volume fraction,
in agreement with Segr\`e et. al. (Phys. Rev. Lett. 79, 2574 (1997)) and the
velocity variance is independent of system size. Detailed predictions are made
for the correlation function in both phases and at the transition.Comment: 4 pages, revtex 1 figur
Molecular Weight Dependence of Spreading Rates of Ultrathin Polymeric Films
We study experimentally the molecular weight dependence of spreading
rates of molecularly thin precursor films, growing at the bottom of droplets of
polymer liquids. In accord with previous observations, we find that the radial
extension R(t) of the film grows with time as R(t) = (D_{exp} t)^{1/2}. Our
data substantiate the M-dependence of D_{exp}; we show that it follows D_{exp}
\sim M^{-\gamma}, where the exponent \gamma is dependent on the chemical
composition of the solid surface, determining its frictional properties with
respect to the molecular transport. In the specific case of hydrophilic
substrates, the frictional properties can be modified by the change of the
relative humidity (RH). We find that \gamma \approx 1 at low RH and tends to
zero when RH gets progressively increased. We propose simple theoretical
arguments which explain the observed behavior in the limits of low and high RH.Comment: 4 pages, 2 figures, to appear in PR
Rhythmogenic neuronal networks, pacemakers, and k-cores
Neuronal networks are controlled by a combination of the dynamics of
individual neurons and the connectivity of the network that links them
together. We study a minimal model of the preBotzinger complex, a small
neuronal network that controls the breathing rhythm of mammals through periodic
firing bursts. We show that the properties of a such a randomly connected
network of identical excitatory neurons are fundamentally different from those
of uniformly connected neuronal networks as described by mean-field theory. We
show that (i) the connectivity properties of the networks determines the
location of emergent pacemakers that trigger the firing bursts and (ii) that
the collective desensitization that terminates the firing bursts is determined
again by the network connectivity, through k-core clusters of neurons.Comment: 4+ pages, 4 figures, submitted to Phys. Rev. Let
Influence of shear flow on vesicles near a wall: a numerical study
We describe the dynamics of three-dimensional fluid vesicles in steady shear
flow in the vicinity of a wall. This is analyzed numerically at low Reynolds
numbers using a boundary element method. The area-incompressible vesicle
exhibits bending elasticity. Forces due to adhesion or gravity oppose the
hydrodynamic lift force driving the vesicle away from a wall. We investigate
three cases. First, a neutrally buoyant vesicle is placed in the vicinity of a
wall which acts only as a geometrical constraint. We find that the lift
velocity is linearly proportional to shear rate and decreases with increasing
distance between the vesicle and the wall. Second, with a vesicle filled with a
denser fluid, we find a stationary hovering state. We present an estimate of
the viscous lift force which seems to agree with recent experiments of Lorz et
al. [Europhys. Lett., vol. 51, 468 (2000)]. Third, if the wall exerts an
additional adhesive force, we investigate the dynamical unbinding transition
which occurs at an adhesion strength linearly proportional to the shear rate.Comment: 17 pages (incl. 10 figures), RevTeX (figures in PostScript
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